Multi-layer biofactory process and precautions

The multi-layer biofactory is a well-designed cell culture system that makes full use of the maximum culture surface in a limited space, thereby saving space; it can be used for industrial-scale production such as vaccines, monoclonal antibodies or biopharmaceuticals, and is particularly suitable for Adherent cells can also be used in suspension culture. The specific operation process of the cell factory is as follows:

multi-layer biofactory

1. Unscrew the lid, and inject the culture medium into the multi-layer biofactory by tilting or pumping. The cell suspension slowly flows into each layer of the cell factory, and the lid is closed and it is still;

2. Slowly stand the multi-layer biofactory on its side, with the side with the liquid filling port facing away from you, and let it stand to make the cell suspension flow into each layer slowly and evenly;

3. Rotate 90 degrees laterally, keep the liquid inlet upward, and let it stand to make the cell suspension flow into each layer slowly and evenly;

4. Hold the side of the liquid inlet with both hands and slowly level it to avoid large shaking of the liquid;

5. Move to a suitable cell culture incubator for culture, make sure to move slowly and steadily, and don't let liquid flow into other layers;

6. After the cultivation, pour the culture medium into the collection container.

Precautions:

1. In order to ensure the uniform distribution of each layer of solution, try to keep it level when moving the multi-layer biofactory;

2. The platform shelf where the multi-layer biofactory is placed must be level to prevent the thickness of the cells on the same layer from being different;

3. When cleaning the cell factory, do not use rapid water flow, otherwise it will cause damage to the cell factory.